Quantify the area under every band
EQBLOT turns Western-blot and In-Cell Western images into defensible numbers — lane detection, background subtraction, peak deconvolution and AUC integration, all in your browser.
From raw gel to publication-ready AUC
Every step of the densitometry pipeline, built for teams.
Lane detection
Automatic detection and grouping of lanes across the membrane, with a distinct overlay so the bands stay easy to read.
Background subtraction
Remove membrane autofluorescence and baseline drift before integration, so every measurement starts from zero.
Peak deconvolution
Resolve overlapping and doublet bands into individual peaks, with per-peak resolution and width.
AUC integration
Integrate the area under each peak with Simpson's rule — per peak or across a whole-lane window.
Reference normalization
Normalize against a loading-control channel to compare samples as relative ratios, not raw intensities.
Team sharing & export
Share experiments across your team and export publication-ready tables and figures in one click.
Four steps to a number you can defend
- 01
Import
Upload 16-bit TIFFs with their metadata — Li-cor Odyssey or generic gels.
- 02
Detect lanes
EQBLOT finds and groups the lanes; adjust the layout whenever you need to.
- 03
Subtract & deconvolve
Remove the background and resolve overlapping bands into clean peaks.
- 04
Integrate & export
Integrate the AUC, normalize to a reference, and export for publication.